Three
Months Industrial Training in Molecular Biology
Hands on training on the following modules:
Isolation Techniques:
> General Technique of Plasmid DNA Isolation
> Preparation of Plasmid DNA by Alkaline Lysis with SDS
> Purification of Plasmid DNA by Chromatography
> Genomic DNA Isoltion
> Isolation of Total RNA
Molecular Cloning:
> Directional Cloning into Plasmid Vectors
> Blunt-ended Cloning into Plasmid Vectors
> Ligating Plasmid and Target DNAs in Low-melting-temperature Agarose
> Preparation and Transformation of Competent E. coli Using Calcium Chloride
> Cloning into Bacteriophage Vectors
Electrophoresis Techniques:
> Detection of DNA in Agarose Gel
> Recovery of DNA from Agarose Gels: Electrophoresis onto DEAE-cellulose Membranes
> Recovery of DNA from Agarose and Polyacrylamide Gels: Electroelution into Dialysis Bags
> Alkaline Agarose Gel Electrophoresis
> Neutral Polyacrylamide Gel Electrophoresis
DNA / RNA Analysis:
> Detection of DNA in Polyacrylamide Gels by Staining
> Restriction Endonuclease Digestion of DNA in Agarose plugs
> Capillary Transfer of DNA to Membranes
> Simultaneous Transfer of DNA from a Single Agrose Gel to Two Membranes
> Purification of RNA from Cells and Tissues by Acid Phenol-Guanidinium Thiocyanate-
Chloroform Extraction
> A Single-step Method for the Simultaneous Preparation of DNA, RNA, and Protein
from Cells and Tissues
Recombinant DNA Technology
Blotting Techniques :
> Western , Southern
Protein Sepration through SDS
> Reducing & Non Reducing
PCR Techniques
> The Basic Polymerase Chain Reaction
> Purification of PCR Products in Preparation for cloning
> Blunt-end Cloning of PCR Products
> Long PCR
> INVERSE PCR
> Quantitative PCR
> GMO Testing
State of art lectures:
> Introduction of molecular biology and its applications
> Lectures on genetic engineering
> Cloning
> Protein Synthesis
> Operon model of DNA regulation
> Lectures on genetic disorders
Three Months Industrial Training in Microbiology
Hands on Training:
General Techniques:
> Preparation and Sterilization of equipment and Culture Media ( GLP )
> Lab QC
> Isolation of microbes from different sources
> Isolation and identification of fungal species
> Sterlity Testing
> Various Staining Techniques
> Pure Culture Preparation
> Spore Counting
Water Testing
Estimation of Bacteriological Condition in :
- Potable Water
- Industrial Water
- Sewage Water
- Trade Water
- Swimming Pool Water
- Trade Waste
Biological Test on Water
- Coli forms
- Total Plate Count
- Pseudomonas
- Streptococci
- E.Coli
- Legionella
Water Analysis
- BOD
- COD
- Odor
- Taste
- Ph
- Turbidity
- Conductivity
- Pesticide Residue etc
Food Testing: Food pathogen screening includes E.Coli , Salmonella , Coliform ,
Yeast & Mold , Anaerobic & Aerobic Plate Count , Aflatoxin etc
Spoilage Analysis
Pathogen Analysis: Proficiency To analyze Food & Water related microorganism
Antimicrobial Effectiveness Test
Endotoxin Test
Fungicidal Activity Test
Virucidal Efficacy Test
Testing of Aerobic Bacteria
Analysis of Denitrifying Bacteria
Acid tolerant Bacteria
Lactic Acid Bacteria Count
Lab Pasteurization Count
Yeast / Mould Count
MIC Test
Sterility Testing
Bioburden Estimation
Testing of Antibiotics
Preservative Efficacy Test
State of Art Lectures :
> Preparation and sterilization of equipment and culture media ( GLP )
> Lab QC
> Quality Audit Tool Kit ( GMP)
> ISO 17001 & HACCP
> NABL
> BIS Standard
> FDA
Projet Work
Three Months Industrial Training in Tissue Culture
Hands on training:
> Sterile techniques and safety procedures
> Preparation of culture media
> Various tissue culture techniques
- Callus culture
- Embryo culture
- Anther culture
- Root culture
> Genetic Manipulations
> Protoplast Culture
> Isolation and purification of secondary metabolites
> Isolation and maintenance of cells
State of art lectures:
> Lectures on Sterile techniques and safety procedures
> Reproduction of rare and extinct species of plants by tissue culture
> Lectures on various methods of hybrid plant production
> Lectures on laboratory and green house procedures
> Role of PTC in biodiversity conservation and economic development
Project Work
Three Months Industrial Training in Bioinformatics
1. Introduction to bioinformatics
> DNA structure
> Protein DNA interaction
> insight to bioinformatics software
> Ramachandran plot
> Basics of internet
2. Computational bioinformatics
Genome sequencing methods
Dynamic programming
Study of algorithms
Statistical significance in biological sequence comparison
3. Genomics
Single nucleotide polymorphism
Human genome project
Sequence formats and databases
Sequence retrieval
Gene structure, repeats
Genetic variations
Gene prediction
Genetic mapping
Sequence alignment
Local sequence alignment method (BLAST)
DNA sequencing
Haplotypes and their significance
Micro array technique
Phred, Phrap and Consed
4. Molecular Modeling
Molecular modeling functions
Genetic algorithms
Conformation analysis
5. Protein Modeling
introduction to docking software
modeling methods
sequence-structure fitness identification
PDB protein structure
Classification of protein structure (CATH, SCOP, FSSP)
Hidden Markov Model
Pattern based similarity search
Reliability of methods for protein structure prediction
6. Introduction to C programming
7. Projet Work
Hands on training on the following modules:
Isolation Techniques:
> General Technique of Plasmid DNA Isolation
> Preparation of Plasmid DNA by Alkaline Lysis with SDS
> Purification of Plasmid DNA by Chromatography
> Genomic DNA Isoltion
> Isolation of Total RNA
Molecular Cloning:
> Directional Cloning into Plasmid Vectors
> Blunt-ended Cloning into Plasmid Vectors
> Ligating Plasmid and Target DNAs in Low-melting-temperature Agarose
> Preparation and Transformation of Competent E. coli Using Calcium Chloride
> Cloning into Bacteriophage Vectors
Electrophoresis Techniques:
> Detection of DNA in Agarose Gel
> Recovery of DNA from Agarose Gels: Electrophoresis onto DEAE-cellulose Membranes
> Recovery of DNA from Agarose and Polyacrylamide Gels: Electroelution into Dialysis Bags
> Alkaline Agarose Gel Electrophoresis
> Neutral Polyacrylamide Gel Electrophoresis
DNA / RNA Analysis:
> Detection of DNA in Polyacrylamide Gels by Staining
> Restriction Endonuclease Digestion of DNA in Agarose plugs
> Capillary Transfer of DNA to Membranes
> Simultaneous Transfer of DNA from a Single Agrose Gel to Two Membranes
> Purification of RNA from Cells and Tissues by Acid Phenol-Guanidinium Thiocyanate-
Chloroform Extraction
> A Single-step Method for the Simultaneous Preparation of DNA, RNA, and Protein
from Cells and Tissues
Recombinant DNA Technology
Blotting Techniques :
> Western , Southern
Protein Sepration through SDS
> Reducing & Non Reducing
PCR Techniques
> The Basic Polymerase Chain Reaction
> Purification of PCR Products in Preparation for cloning
> Blunt-end Cloning of PCR Products
> Long PCR
> INVERSE PCR
> Quantitative PCR
> GMO Testing
State of art lectures:
> Introduction of molecular biology and its applications
> Lectures on genetic engineering
> Cloning
> Protein Synthesis
> Operon model of DNA regulation
> Lectures on genetic disorders
Three Months Industrial Training in Microbiology
Hands on Training:
General Techniques:
> Preparation and Sterilization of equipment and Culture Media ( GLP )
> Lab QC
> Isolation of microbes from different sources
> Isolation and identification of fungal species
> Sterlity Testing
> Various Staining Techniques
> Pure Culture Preparation
> Spore Counting
Water Testing
Estimation of Bacteriological Condition in :
- Potable Water
- Industrial Water
- Sewage Water
- Trade Water
- Swimming Pool Water
- Trade Waste
Biological Test on Water
- Coli forms
- Total Plate Count
- Pseudomonas
- Streptococci
- E.Coli
- Legionella
Water Analysis
- BOD
- COD
- Odor
- Taste
- Ph
- Turbidity
- Conductivity
- Pesticide Residue etc
Food Testing: Food pathogen screening includes E.Coli , Salmonella , Coliform ,
Yeast & Mold , Anaerobic & Aerobic Plate Count , Aflatoxin etc
Spoilage Analysis
Pathogen Analysis: Proficiency To analyze Food & Water related microorganism
Antimicrobial Effectiveness Test
Endotoxin Test
Fungicidal Activity Test
Virucidal Efficacy Test
Testing of Aerobic Bacteria
Analysis of Denitrifying Bacteria
Acid tolerant Bacteria
Lactic Acid Bacteria Count
Lab Pasteurization Count
Yeast / Mould Count
MIC Test
Sterility Testing
Bioburden Estimation
Testing of Antibiotics
Preservative Efficacy Test
State of Art Lectures :
> Preparation and sterilization of equipment and culture media ( GLP )
> Lab QC
> Quality Audit Tool Kit ( GMP)
> ISO 17001 & HACCP
> NABL
> BIS Standard
> FDA
Projet Work
Three Months Industrial Training in Tissue Culture
Hands on training:
> Sterile techniques and safety procedures
> Preparation of culture media
> Various tissue culture techniques
- Callus culture
- Embryo culture
- Anther culture
- Root culture
> Genetic Manipulations
> Protoplast Culture
> Isolation and purification of secondary metabolites
> Isolation and maintenance of cells
State of art lectures:
> Lectures on Sterile techniques and safety procedures
> Reproduction of rare and extinct species of plants by tissue culture
> Lectures on various methods of hybrid plant production
> Lectures on laboratory and green house procedures
> Role of PTC in biodiversity conservation and economic development
Project Work
Three Months Industrial Training in Bioinformatics
1. Introduction to bioinformatics
> DNA structure
> Protein DNA interaction
> insight to bioinformatics software
> Ramachandran plot
> Basics of internet
2. Computational bioinformatics
Genome sequencing methods
Dynamic programming
Study of algorithms
Statistical significance in biological sequence comparison
3. Genomics
Single nucleotide polymorphism
Human genome project
Sequence formats and databases
Sequence retrieval
Gene structure, repeats
Genetic variations
Gene prediction
Genetic mapping
Sequence alignment
Local sequence alignment method (BLAST)
DNA sequencing
Haplotypes and their significance
Micro array technique
Phred, Phrap and Consed
4. Molecular Modeling
Molecular modeling functions
Genetic algorithms
Conformation analysis
5. Protein Modeling
introduction to docking software
modeling methods
sequence-structure fitness identification
PDB protein structure
Classification of protein structure (CATH, SCOP, FSSP)
Hidden Markov Model
Pattern based similarity search
Reliability of methods for protein structure prediction
6. Introduction to C programming
7. Projet Work
