Six Months
Advanced Hands on Training in Biotechnology With Project Work
Our Pedalogy
> Class room lectures
> Presentations
> Hands on training
> Group Discussion
> Project Work
We will cover the following modules in six months training:
Module: I ( Microbial Techniques )
> Preparation and Sterilisation of equipment and Culture Media ( GLP )
> Lab QC
> Isolation of culture media from different sources
> Isolation and identification of fungal species
> Mirobial Testing of Water & Food ( BIS Std.) and Maintenance of Data
> Sterlity Testing
> Pure Culture Preparation
> Spore Counting
> Quality Audit Tool Kit
> Spoilage Analysis
> Pathogen Analysis: Proficiency To analyze Food & Water related microorganism
> Antimicrobial Effectiveness Test
> Endotoxin Test
> Fungicidal Activity Test
> Virucidal Efficacy Test
> Testing of Aerobic Bacteria
> Analysis of Denitrifying Bacteria
> Acid tolerant Bacteria
> Lactic Acid Bacteria Count
> Lab Pasteurization Count
> Yeast / Mould Count
> MIC Test
> Bioburden Estimation
> Testing of Antibiotics
> Preservative Efficacy Test
Module: II ( Molecular Biology )
Isolation Techniques:
> General Technique of Plasmid DNA Isolation
> Preparation of Plasmid DNA
> Purification of Plasmid DNA by Chromatography
> Genomic DNA isolation
> Isolation of Total RNA
Molecular Cloning:
> Directional Cloning into Plasmid Vectors
> Blunt-ended Cloning into Plasmid Vectors
> Performing Plasmid Ligation
> Preparation and Transformation of Competent E. coli Using various techniques
> Cloning into Bacteriophage Vectors
Electrophoresis Techniques:
> Recovery of DNA from Agarose Gels: Electrophoresis onto DEAE-cellulose Membranes
> Recovery of DNA from Agarose and Polyacrylamide Gels: Electroelution into Dialysis Bags
> Alkaline Agarose Gel Electrophoresis
> Neutral Polyacrylamide Gel Electrophoresis
DNA / RNA Analysis
> Restriction Endonuclease Digestion of DNA in Agarose plugs
> Capillary Transfer of DNA to Membranes
> Simultaneous Transfer of DNA from a Single Agrose Gel to Two Membranes
> Purification of RNA from Cells and Tissues by Acid Phenol-Guanidinium Thiocyanate- Chloroform Extraction
> A Single-step Method for the Simultaneous Preparation of DNA, RNA, and Protein from Cells and Tissues
> Recombinant DNA Technology
> Blotting Techniques : Western, Southern, northern
> Protein Separation through SDS-PAGE
PCR Techniques
> Purification of PCR Products in Preparation for cloning
> Blunt-end Cloning of PCR Products
> Long PCR
> INVERSE PCR
> Quantitative PCR
Module: III ( Tissue Culture )
> Sterile techniques and safety procedures
> Preparation of media for tissue culture
> Initiation and maintenance of primary cell culture and suspension cell lines
- Anther Culture
- Root Culture
- Callus Culture
> Isolation & Maintenance of cells in culture
> Toxicology testing
Module: IV ( Immunology )
> Lymphoid Cell Culture Derived From Blood
> Lymphoid Cell Count
> ELISA
> Performing various Staining procedures
> Immuno Electrophoresis
Module: V ( Bioinformatics)
Basic knowledge of Bioinformatics Databases , Tools and Analysis
Module: VI ( IPR )
> Patent Law & Procedure
> Patent Specification Drafting
> Patent Searching
> Drafting of other patent related documents
Module : VII State of art lectures:
> Preparation and sterilization of equipment and culture media ( GLP )
> Inoculation techniques
> Laboratory Quality Control
> Lectures on BIS Standards
> Introduction of molecular biology and its applications
> Lectures on genetic engineering
> Cloning
> Protein Synthesis
> Lectures on genetic disorders
> Lectures on complement system
> Lectures on immune response to various diseases
> Cancer and immune system
> Biotechnology and patents
Projet Work : It is mandatory to handle a project in each module.
Write Us:
allelelifesciences@gmail.com
Our Pedalogy
> Class room lectures
> Presentations
> Hands on training
> Group Discussion
> Project Work
We will cover the following modules in six months training:
Module: I ( Microbial Techniques )
> Preparation and Sterilisation of equipment and Culture Media ( GLP )
> Lab QC
> Isolation of culture media from different sources
> Isolation and identification of fungal species
> Mirobial Testing of Water & Food ( BIS Std.) and Maintenance of Data
> Sterlity Testing
> Pure Culture Preparation
> Spore Counting
> Quality Audit Tool Kit
> Spoilage Analysis
> Pathogen Analysis: Proficiency To analyze Food & Water related microorganism
> Antimicrobial Effectiveness Test
> Endotoxin Test
> Fungicidal Activity Test
> Virucidal Efficacy Test
> Testing of Aerobic Bacteria
> Analysis of Denitrifying Bacteria
> Acid tolerant Bacteria
> Lactic Acid Bacteria Count
> Lab Pasteurization Count
> Yeast / Mould Count
> MIC Test
> Bioburden Estimation
> Testing of Antibiotics
> Preservative Efficacy Test
Module: II ( Molecular Biology )
Isolation Techniques:
> General Technique of Plasmid DNA Isolation
> Preparation of Plasmid DNA
> Purification of Plasmid DNA by Chromatography
> Genomic DNA isolation
> Isolation of Total RNA
Molecular Cloning:
> Directional Cloning into Plasmid Vectors
> Blunt-ended Cloning into Plasmid Vectors
> Performing Plasmid Ligation
> Preparation and Transformation of Competent E. coli Using various techniques
> Cloning into Bacteriophage Vectors
Electrophoresis Techniques:
> Recovery of DNA from Agarose Gels: Electrophoresis onto DEAE-cellulose Membranes
> Recovery of DNA from Agarose and Polyacrylamide Gels: Electroelution into Dialysis Bags
> Alkaline Agarose Gel Electrophoresis
> Neutral Polyacrylamide Gel Electrophoresis
DNA / RNA Analysis
> Restriction Endonuclease Digestion of DNA in Agarose plugs
> Capillary Transfer of DNA to Membranes
> Simultaneous Transfer of DNA from a Single Agrose Gel to Two Membranes
> Purification of RNA from Cells and Tissues by Acid Phenol-Guanidinium Thiocyanate- Chloroform Extraction
> A Single-step Method for the Simultaneous Preparation of DNA, RNA, and Protein from Cells and Tissues
> Recombinant DNA Technology
> Blotting Techniques : Western, Southern, northern
> Protein Separation through SDS-PAGE
PCR Techniques
> Purification of PCR Products in Preparation for cloning
> Blunt-end Cloning of PCR Products
> Long PCR
> INVERSE PCR
> Quantitative PCR
Module: III ( Tissue Culture )
> Sterile techniques and safety procedures
> Preparation of media for tissue culture
> Initiation and maintenance of primary cell culture and suspension cell lines
- Anther Culture
- Root Culture
- Callus Culture
> Isolation & Maintenance of cells in culture
> Toxicology testing
Module: IV ( Immunology )
> Lymphoid Cell Culture Derived From Blood
> Lymphoid Cell Count
> ELISA
> Performing various Staining procedures
> Immuno Electrophoresis
Module: V ( Bioinformatics)
Basic knowledge of Bioinformatics Databases , Tools and Analysis
Module: VI ( IPR )
> Patent Law & Procedure
> Patent Specification Drafting
> Patent Searching
> Drafting of other patent related documents
Module : VII State of art lectures:
> Preparation and sterilization of equipment and culture media ( GLP )
> Inoculation techniques
> Laboratory Quality Control
> Lectures on BIS Standards
> Introduction of molecular biology and its applications
> Lectures on genetic engineering
> Cloning
> Protein Synthesis
> Lectures on genetic disorders
> Lectures on complement system
> Lectures on immune response to various diseases
> Cancer and immune system
> Biotechnology and patents
Projet Work : It is mandatory to handle a project in each module.
Write Us:
allelelifesciences@gmail.com
